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Sr Research Technologist

Company: St. Jude Children's Research Hospital
Location: Raleigh
Posted on: September 16, 2020

Job Description:

With resources to help you succeed and develop your career, we also offer generous relocation assistance.The Kriwacki lab seeks to understand how phase separation drives the condensation of biomolecules to create liquid-like bodies that mediate diverse biological processes. Importantly, aberrant biomolecular condensation is associated with several devastating human diseases, including cancer and neurodegeneration. A new initiative in the laboratory addresses the role of aberrant phase separation by fusion oncoproteins as drivers in cancer (e.g., NUP98 fusions in AML & AEL). We use an integrative approach, involving a combination of cell and molecular biology, biophysical, and live and fixed cell imaging techniques, coupled with computational methods, to determine how phase separation by NUP98 fusion oncoproteins contributes to the formation of aberrant transcription centers that drive aberrant gene expression and transform hematopoietic cells. We seek an analytical and self-motivated researcher to join the team as a Sr Research Technologist.The Sr. Research Technologist will be involved in the following responsibilities:Perform mammalian cell culture experiments using proper aseptic technique.Perform serial colony formation assays using hematopoietic stem cells transformed with vectors expressing various types of fusion oncoproteins.Prepare transformed cells for analysis of gene expression using qPCR, and chromatin structure and function using methods including RNA-seq, ChIP-seq, and HiC. Perform mammalian gene cloning from primary cancer cells. Display excellent quantitative, communication and record keeping skills. Prior experience with methods used to study cancer genomics and/or epigenetics is desired. Operate as an advanced-level Senior Research Technologist by assisting the principal investigator with data collection and analysis for research projects.Properly conducts specific projects, maintains efficient laboratory operations and may assist in training other laboratory personnel. Performs other duties as assigned or directed in order to meet the goals and objectives of the department.Bachelor's degree in an appropriate scientific field is required.The ideal candidate will have a M.S. or Ph.D. degree in biochemistry, molecular biology, structural biology, biophysics or related discipline and have experience with molecular biology, protein biochemistry and biophysical methods for protein characterization. Candidates with a B.S. degree and relevant professional experience will also be considered. A minimum of three (3) years total work experience as a SJCRH Research Technologist or three (3) years relevant post-degree experience in a similar research environment is required with a Bachelor's degree.A minimum of two (2) years total work experience as a SJCRH Research Technologist or two (2) years relevant post-degree experience in a similar research environment is required with a Master's degree.No experience is required with a PhD in a related field.St. Jude is an Equal Opportunity EmployerNo Search Firms:St. Jude Children's Research Hospital does not accept unsolicited assistance from search firms for employment opportunities. Please do not call or email. All resumes submitted by search firms to any employee or other representative at St. Jude via email, the internet or in any form and/or method without a valid written search agreement in place and approved by HR will result in no fee being paid in the event the candidate is hired by St. Jude.

Keywords: St. Jude Children's Research Hospital, Raleigh , Sr Research Technologist, Other , Raleigh, North Carolina

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